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The pathogen spread to 12 new districts in the following years with varying level of devastation (Surovy et al. By collecting blast-infected plant samples and analysing with a field pathogenomics approach, we found that wheat blast in Bangladesh was caused by a South American lineage of M. The disease severity and associated yield loss varied widely among these different districts (Islam et al. Out of a total 101,660 ha of cultivated wheat in those eight districts, an estimated 15% were affected by wheat blast. Wheat blast appeared for the first time in Bangladesh in February 2016 and caused up to 100% yield losses in eight districts of Southwestern part of Bangladesh (Islam et al. 1993) and neighbouring countries including Bolivia, Argentina and Paraguay within a few years (Barea and Toledo 1996 Viedma 2005). Immediately after that the disease spread fast and was found all over the wheat-growing regions in Brazil (Urashima et al. oryzae Triticum ( MoT) and was first reported in Paraná state of Brazil in 1985 (Igarashi et al. The pathotype that infects wheat is known as M.
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Successful characterization of MoT isolates at morphological and molecular levels coupled with detection of the pathogen in infected field and seed lots should be useful for efficient surveillance and management of the fearsome wheat blast disease.īlast disease caused by Magnaporthe oryzae (Hebert) Barr (anamorph Pyricularia oryzae) is the economically most important fungal disease of rice, wheat, barley, millet, oat and many other plants of Poaceae family (Valent et al.
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This method was also proven to be more user-friendly and cost-effective than previously available methods. Additionally, the simple method we developed in our study for producing abundant conidia in a very short period of time will be very helpful in studying biology of the wheat blast fungus. We also successfully established a PCR-based detection system based on a commercially available detection kit for field-infected leaf and seed samples by detecting Pot2- and MoT3-specific bands. oryzae-specific primers Pot1 and Pot2 and MoT-specific primers MoT3F and MoT3R produced bands with the expected size from all wheat-infecting isolates. Cultural and morphological characterization of the isolates revealed that all Bangladeshi MoT isolates are of a single clonal lineage with similar cultural and morphological characters. We developed suitable methods for isolation of MoT from field-infected plant samples using modified monoconidial isolation technique and produced abundant conidia from a single mycelial plate for in vivo pathogenicity assay in a reproducible manner. oryzae or de novo method development and validation. This process included modification of existing protocols that were available for a related fungal pathogen M. In this study, we aimed to develop suitable methods for isolation, storage and morphological characterization and molecular identification of MoT isolates collected from the blast-infected wheat fields in Bangladesh. Efficient and suitable methods for isolation, storage, inoculum production and molecular characterization of the pathogen can help in achieving the target of sustainable management of the disease in a relatively short period of time. Wheat blast disease caused by a South American lineage of Magnaporthe oryzae Triticum ( MoT) pathotype has emerged as a serious threat to wheat production in Bangladesh since its first emergence in 2016.